ARG82071

Human ESM1 ELISA Kit

Human ESM1 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82071-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82071-002 Standard 2 X 10 ng/vial 4°C
ARG82071-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82071-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82071-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82071-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82071-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82071-008 25X Wash buffer 20 ml 4°C
ARG82071-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82071-010 STOP solution 10 ml (Ready to use) 4°C
ARG82071-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82071 Human ESM1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ESM1 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name ESM1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.3%; Inter-Assay CV: 7.2%
Alternate Names endocan; Endothelial cell-specific molecule 1; ESM-1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11082 Human ESM1

Swiss-port # Q9NQ30 Human Endothelial cell-specific molecule 1

Gene Symbol ESM1
Gene Full Name endothelial cell-specific molecule 1
Background This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
Function Involved in angiogenesis; promotes angiogenic sprouting. May have potent implications in lung endothelial cell-leukocyte interactions. [UniProt]
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PTM May contain intrachain disulfide bonds.

O-glycosylated; contains chondroitin sulfate and dermatan sulfate. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG82071 Human ESM1 ELISA Kit standard curve image

    ARG82071 Human ESM1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG82071 Human ESM1 ELISA Kit User's manual download icon Download