ARG81570
Human ERBB3 / HER3 ELISA Kit
Human ERBB3 / HER3 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81570-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81570-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81570-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81570-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81570-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81570-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81570-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81570-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81570-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81570-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81570-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81570 Human ERBB3 / HER3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ERBB3 / HER3 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | ERBB3 / HER3 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.8% Inter-Assay CV: 6.8% |
| Alternate Names | MDA-BF-1; LCCS2; p180-ErbB3; Proto-oncogene-like protein c-ErbB-3; c-erbB3; p85-sErbB3; Tyrosine kinase-type cell surface receptor HER3; p45-sErbB3; erbB3-S; HER3; c-erbB-3; ErbB-3; EC 2.7.10.1; Receptor tyrosine-protein kinase erbB-3 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P21860 Human Receptor tyrosine-protein kinase erbB-3 |
|---|---|
| Gene Symbol | ERBB3 |
| Gene Full Name | erb-b2 receptor tyrosine kinase 3 |
| Background | This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq, Jul 2008] |
| Function | Binds and is activated by neuregulins and NTAK. May also be activated by CSPG5. [UniProt] |
| Highlight | Related products: ERBB3 antibodies; ERBB3 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Autophosphorylated (PubMed:20351256). Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed:20682778). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81570 Human ERBB3 / HER3 ELISA Kit standard curve image
ARG81570 Human ERBB3 / HER3 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81570 Human ERBB3 / HER3 ELISA Kit User's manual |
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