ARG81621
Human ENPP2 ELISA Kit
Human ENPP2 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81621-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81621-002 | Standard | 2 X 40 ng/vial | 4°C |
| ARG81621-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81621-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81621-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81621-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81621-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81621-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81621-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81621-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81621-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81621 Human ENPP2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ENPP2 in serum, plasma (heparin), urine, human milk and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | ENPP2 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 312.5 pg/ml |
| Sample Type | Serum, plasma (heparin), urine, human milk and cell culture supernatants. |
| Standard Range | 625 - 40000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.3% Inter-Assay CV: 6.7% |
| Alternate Names | Ectonucleotide pyrophosphatase/phosphodiesterase family member 2; Extracellular lysophospholipase D; PDNP2; NPP2; AUTOTAXIN; ATX-X; Autotaxin; LysoPLD; EC 3.1.4.39; PD-IALPHA; E-NPP 2; ATX |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13822 Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 |
|---|---|
| Gene Symbol | ENPP2 |
| Gene Full Name | ectonucleotide pyrophosphatase/phosphodiesterase 2 |
| Background | The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2008] |
| Function | Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor. [UniProt] |
| Highlight | Related products: ENPP2 antibodies; ENPP2 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N-glycosylation, but not furin-cleavage, plays a critical role on secretion and on lysoPLD activity. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81621 Human ENPP2 ELISA Kit standard curve image
ARG81621 Human ENPP2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81621 Human ENPP2 ELISA Kit User's manual |
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