ARG80137
Human EGF ELISA Kit
Human EGF ELISA Kit for ELISA and Human
Cancer kit; Cell Biology and Cellular Response kit; Signaling Transduction kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80137-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80137-002 | Standard (Lyophilized) | 3 X 1 ng/vial | 4°C |
| ARG80137-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80137-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80137-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80137-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80137-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80137-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80137-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80137-010 | STOP solution | 12ml | 4°C |
| ARG80137-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80137 Human EGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human EGF in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human Amphiregulin, Betacellulin, EGFR, Epigen, Epiregulin, ErbB2,3,4, HB-EGF, NRG1/HRG1, NRG1 Isoform SMDF, NRG1- alpha/HRG1- alpha EGF Domain, NRG1- beta1/HRG1- beta1 EGF Domain, NRG1- beta1/HRG1- beta1 ECD, TNF- alpha |
| Target Name | EGF |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 4 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 7.8 - 500 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: <10% |
| Alternate Names | Urogastrone; Pro-epidermal growth factor; URG; HOMG4; EGF |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | EGF |
| Gene Full Name | epidermal growth factor |
| Background | This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010] |
| Function | EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). [UniProt] |
| Highlight | Related products: EGF antibodies; EGF ELISA Kits; EGF Duos / Panels; EGF recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cancer kit; Cell Biology and Cellular Response kit; Signaling Transduction kit |
| PTM | O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). |
Images (1) Click the Picture to Zoom In
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ARG80137 Human EGF ELISA Kit standard curve image
ARG80137 Human EGF ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80137 Human EGF ELISA Kit User's manual |
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