ARG82365
Human Dermatopontin ELISA Kit
Human Dermatopontin ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82365-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82365-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82365-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82365-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82365-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82365-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82365-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82365-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82365-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82365-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82365-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82365 Human Dermatopontin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Dermatopontin in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Dermatopontin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 46.85 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 93.7 - 6000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.9% Inter-Assay CV: 7.6% |
| Alternate Names | TRAMP; Tyrosine-rich acidic matrix protein; Dermatopontin |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DPT |
| Gene Full Name | dermatopontin |
| Background | Dermatopontin is an extracellular matrix protein with possible functions in cell-matrix interactions and matrix assembly. The protein is found in various tissues and many of its tyrosine residues are sulphated. Dermatopontin is postulated to modify the behavior of TGF-beta through interaction with decorin. [provided by RefSeq, Jul 2008] |
| Function | Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation (By similarity). [UniProt] |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. [UniProt] |
| Highlight | Related products: Dermatopontin antibodies; Dermatopontin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Sulfated on tyrosine residue(s). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82365 Human Dermatopontin ELISA Kit standard curve image
ARG82365 Human Dermatopontin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82365 Human Dermatopontin ELISA Kit User manual |
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