ARG81449

Human Decorin ELISA Kit

Human Decorin ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81449-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81449-002 Standard 2 X 10 ng/vial 4°C
ARG81449-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81449-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81449-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81449-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81449-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81449-008 25X Wash buffer 20 ml 4°C
ARG81449-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81449-010 STOP solution 10 ml (Ready to use) 4°C
ARG81449-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81449 Human Decorin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Decorin in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Decorin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 7.1%
Inter-Assay CV: 7.8%
Alternate Names Bone proteoglycan II; PG40; PGII; CSCD; SLRR1B; PGS2; PG-S2; DSPG2; Decorin

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1634 Human DCN

Swiss-port # P07585 Human Decorin

Gene Symbol DCN
Gene Full Name decorin
Background The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to biglycan protein. The encoded protein and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. It contains one attached glycosaminoglycan chain. This protein is capable of suppressing the growth of various tumor cell lines. There are multiple alternatively spliced transcript variants known for this gene. This gene is a candidate gene for Marfan syndrome. [provided by RefSeq, Jul 2008]
Function May affect the rate of fibrils formation. [UniProt]
Highlight Related products:
Decorin antibodies; Decorin ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM The attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG81449 Human Decorin ELISA Kit standard curve image

    ARG81449 Human Decorin ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81449 Human Decorin ELISA Kit User's manual download icon Download