ARG81609
Human DR3 / TNFRSF25 ELISA Kit
Human DR3 / TNFRSF25 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81609-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81609-002 | Standard | 2 X 20 ng/vial | 4°C |
| ARG81609-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81609-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81609-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81609-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81609-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81609-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81609-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81609-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81609-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81609 Human DR3 / TNFRSF25 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human DR3 / TNFRSF25 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | DR3 / TNFRSF25 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 156 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 312 - 20000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.5% Inter-Assay CV: 7.0% |
| Alternate Names | WSL-1; Apo-3; Apoptosis-mediating receptor TRAMP; DDR3; DR3; Protein WSL; Protein WSL-1; TRAMP; Apoptosis-inducing receptor AIR; TNFRSF12; Death receptor 3; LARD; Apoptosis-mediating receptor DR3; TR3; APO-3; Lymphocyte-associated receptor of death; Tumor necrosis factor receptor superfamily member 25; WSL-LR |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q93038 Human Tumor necrosis factor receptor superfamily member 25 |
|---|---|
| Gene Symbol | TNFRSF25 |
| Gene Full Name | tumor necrosis factor receptor superfamily, member 25 |
| Background | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed preferentially in the tissues enriched in lymphocytes, and it may play a role in regulating lymphocyte homeostasis. This receptor has been shown to stimulate NF-kappa B activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domain containing adaptor proteins. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation. [provided by RefSeq, Jul 2008] |
| Function | Receptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis. [UniProt] |
| Highlight | Related products: TNFRSF25 antibodies; TNFRSF25 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
-
ARG81609 Human DR3 / TNFRSF25 ELISA Kit standard curve image
ARG81609 Human DR3 / TNFRSF25 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81609 Human DR3 / TNFRSF25 ELISA Kit User's manual |
 Download
|
