ARG81554
Human DKK3 / REIC ELISA Kit
Human DKK3 / REIC ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81554-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81554-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81554-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81554-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81554-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81554-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81554-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81554-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81554-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81554-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81554-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81554 Human DKK3 / REIC ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human DKK3 / REIC in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | DKK3 / REIC |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.7% Inter-Assay CV: 6.5% |
| Alternate Names | Dickkopf-3; REIC; Dkk-3; RIG; hDkk-3; Dickkopf-related protein 3 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DKK3 |
| Gene Full Name | dickkopf WNT signaling pathway inhibitor 3 |
| Background | This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008] |
| Function | Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity). [UniProt] |
| Highlight | Related products: DKK3 antibodies; DKK3 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N- and O-glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81554 Human DKK3 / REIC ELISA Kit standard curve image
ARG81554 Human DKK3 / REIC ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81554 Human DKK3 / REIC ELISA Kit User's manual |
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