ARG80949
Human DHEA ELISA Kit
Human DHEA ELISA Kit for ELISA and Human
Signaling Transduction kit
Overview
| Product Description | ARG80949 DHEA ELISA Kit is an Enzyme Immunoassay kit for the quantification of DHEA in human serum and plasma (EDTA). |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | DHEA |
| Conjugation | HRP |
| Sensitivity | 0.07 ng/ml |
| Sample Type | Serum and plasma (EDTA). |
| Standard Range | 0.3 - 30 ng/ml |
| Sample Volume | 25 μl |
Application Instructions
| Assay Time | 1 h (RT/shaker), 30 min (dark) |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | Dehydroepiandrosterone (DHEA; androstenolone; 3b-hydroxy-5-androsten-17-one) is a C19 steroid produced in the adrenal cortex and, to a lesser extent, gonads. DHEA serves as a precursor in testosterone and estrogen synthesis. Due to the presence of a 17-oxo (rather than hydroxyl) group, DHEA has relatively weak androgenic activity, which has been estimated at ~10% that of testosterone. However in neonates, peripubertal children and in adult women, circulating DHEA levels may be several-fold higher than testosterone concentrations, and rapid peripheral tissue conversion to more potent androgens (androstenedione and testosterone) and estrogens may occur. Moreover, DHEA has relatively low affinity for sex-hormone binding globulin. These factors may enhance the physiologic biopotency of DHEA. |
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| Function | The physiologic role of DHEA has not been conclusively defined. A variety of in vitro effects, including antiproliferative effects in different cell lines and effects on enzyme-mediated cell metabolism, have been reported. In vivo studies suggest that DHEA may affect cholesterol and lipid metabolism, insulin sensitivity and secretion and immune function. Abnormal DHEA levels have been reported in schizophrenia and obesity. Therapeutic administration of DHEA has been proposed for several conditions, including obesity and cardiovascular disease. |
| Highlight | Related products: DHEA antibodies; DHEA ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Signaling Transduction kit |
Images (1) Click the Picture to Zoom In
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ARG80949 Human DHEA ELISA Kit standard curve image
ARG80949 Human DHEA ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG80949 Human DHEA ELISA Kit User manual |
 Download
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