ARG82619
Human DBP / Vitamin D binding protein ELISA Kit
Human DBP / Vitamin D binding protein ELISA Kit for ELISA and Human
Overview
| Product Description | ARG82619 Human DBP / Vitamin D binding protein ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human DBP in serum, plasma, cell culture supernatants, urine, saliva, milk and cerebrospinal fluid. |
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| Tested Reactivity | Hu |
| Species Does Not React With | Ms, Rat, Bov, Dog, Mk, Pig, Rb |
| Tested Application | ELISA |
| Specificity | The kit does not cross-react with the following species: Dog, Bovine, Monkey, Mouse, Rat, Pig and Rabbit. |
| Target Name | DBP / Vitamin D binding protein |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.38 ng/ml |
| Sample Type | Serum, plasma, cell culture supernatants, urine, saliva, milk and cerebrospinal fluid. |
| Standard Range | 1.56 - 100 ng/ml |
| Sample Volume | 50 µl |
| Precision | Intra-Assay CV: 4.7% Inter-Assay CV: 9.2% |
| Alternate Names | GRD3; DBP/GC; HEL-S-51; VDBG; VDB; Gc-globulin; DBP; VDBP; Vitamin D-binding protein; Group-specific component |
Application Instructions
| Assay Time | ~ 4 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GC |
| Gene Full Name | group-specific component (vitamin D binding protein) |
| Background | The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011] |
| Function | Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: DBP antibodies; DBP ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Allele GC*1S is O-glycosylated at Thr-436. The trisaccharide sugar moiety can be modified by the successive removal of neuraminic acid and galactose leaving an O-linked N-acetyl-galactosamine. This conversion is thought to produce a macrophage-activating factor (Gc-MAF). Only a minor proportion of plasma GC is O-glycosylated. The potential N-glycosylation site predicted at Asn-288 is thought to be nonglycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82619 Human DBP / Vitamin D binding protein ELISA Kit standard curve image
ARG82619 Human DBP / Vitamin D binding protein ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82619 Human DBP / Vitamin D binding protein ELISA Kit User manual |
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