ARG81620
Human Cystatin SN ELISA Kit
Human Cystatin SN ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81620-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81620-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81620-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81620-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81620-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81620-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81620-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81620-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81620-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81620-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81620-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81620 Human Cystatin SN ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Cystatin SN in serum, plasma (heparin, EDTA), saliva, urine and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Cystatin SN |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), saliva, urine and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.7% Inter-Assay CV: 8.0% |
| Alternate Names | Salivary cystatin-SA-1; Cystain-SA-I; Cystatin-1; Cystatin-SN |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CST1 |
| Gene Full Name | cystatin SN |
| Background | The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a cysteine proteinase inhibitor found in saliva, tears, urine, and seminal fluid. [provided by RefSeq, Jul 2008] |
| Function | Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Cystatin SN, with a pI of 7.5, is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin S, although both inhibit ficin equally well. [UniProt] |
| Highlight | Related products: Cystatin antibodies; Cystatin ELISA Kits; Cystatin Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
-
ARG81620 Human Cystatin SN ELISA Kit standard curve image
ARG81620 Human Cystatin SN ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81620 Human Cystatin SN ELISA Kit User manual |
 Download
|
