ARG82068
Human Cystatin B / Stefin B ELISA Kit
Human Cystatin B / Stefin B ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82068-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82068-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82068-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82068-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82068-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82068-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82068-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82068-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82068-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82068-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82068-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | Human Cystatin B / Stefin B ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Cystatin B / Stefin B in serum, plasma (heparin, EDTA), saliva, urine, human milk and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Cystatin B / Stefin B |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), saliva, urine, human milk and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 7.4%; Inter-Assay CV: 8.2% |
| Alternate Names | Liver thiol proteinase inhibitor; EPM1; CPI-B; EPM1A; Cystatin-B; Stefin-B; PME; CST6; ULD; STFB |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CSTB |
| Gene Full Name | cystatin B (stefin B) |
| Background | The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). [provided by RefSeq, Jul 2008] |
| Function | This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B. [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. [UniProt] |
| Highlight | Related products: Cystatin antibodies; Cystatin ELISA Kits; Cystatin Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82068 Human Cystatin B / Stefin B ELISA Kit standard curve image
ARG82068 Human Cystatin B / Stefin B ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82068 Human Cystatin B / Stefin B ELISA Kit User's manual |
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