ARG83301
Human Cpn 10 ELISA Kit
Human Cpn 10 ELISA Kit for ELISA and Human
Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | ARG83301 Human Cpn 10 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Cpn 10 in Serum, Plasma and Cell culture supernatants. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Cpn 10 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 75 pg/ml |
| Detection Range | 156 pg/ml - 10,000 pg/ml |
| Sample Type | Serum, Plasma and Cell culture supernatants |
| Precision | Intra-Assay CV: 5.7% Inter-Assay CV: 4.8% |
| Alternate Names | HSP10; 10 kDa chaperonin; CPN10; Early-pregnancy factor; Hsp10; GROES; EPF; Chaperonin 10; 10 kDa heat shock protein, mitochondrial |
Application Instructions
| Assay Time | ~ 5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P61604 Human 10 kDa heat shock protein, mitochondrial |
|---|---|
| Gene Symbol | HSPE1 |
| Gene Full Name | heat shock 10kDa protein 1 |
| Background | Chaperonin 10, otherwise known as Cpn10, (groES in E.coli) make up a family of small heart shock proteins with an approximate molecular mass of 10kDa (Hsp10s). Cpn10 acts as a co-chaperone and interacts with the Hsp60 family to promote proper folding of polypeptides. Cpn10 and Cpn60 both exhibit sevenfold axis of symmetry and function as a team in the protein folding and assembly process. Cpn10 has been located in human platelets, but is also present in human maternal serum. It has been reported that human Cpn10 is identical with early pregnancy factor, which is involved in control over cell growth and development. This identification suggest that Cpn10 may act like a hormone in stressful situations such as pregnancy. |
| Function | Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. [UniProt] |
| Cellular Localization | Mitochondrion Matrix |
| Research Area | Metabolism antibody; Signaling Transduction antibody |
Images (1) Click the Picture to Zoom In
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ARG83301 Human Cpn 10 ELISA Kit standard curve image
ARG83301 Human Cpn 10 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83301 Human Cpn 10 ELISA Kit User manual |
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