ARG82648
Human Complement Factor B ELISA Kit
Human Complement Factor B ELISA Kit for ELISA and Human
Overview
| Product Description | ARG82648 Human Complement Factor B ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Complement Factor B in serum, plasma, cell culture supernatants, saliva, milk, cell lysate and CSF. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | Cross-Reactivity: Monkey: < 5% Mouse, Rat, Pig, Dog, Bovine and Rabbit: None No significant cross-reactivity observed with complement factor D, factor H, factor I, factor P, C1, C2, C3, C4, C5, C6, C7, C8 and C9. 10% FBS in culture media will not affect the assay. |
| Target Name | Complement Factor B |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 1.1 ng/ml |
| Sample Type | Serum, plasma, cell culture supernatants, saliva, milk, cell lysate and CSF. |
| Standard Range | 2.188 - 140 ng/ml |
| Sample Volume | 50 µl |
| Precision | Intra-Assay CV: 5.1% Inter-Assay CV: 9.9% |
| Alternate Names | Properdin factor B; GBG; BF; PBF2; CFAB; BFD; Complement factor B; CFBD; Glycine-rich beta glycoprotein; FB; ARMD14; C3/C5 convertase; EC 3.4.21.47; FBI12; AHUS4; H2-Bf |
Application Instructions
| Assay Time | ~ 4 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | CFB |
| Gene Full Name | complement factor B |
| Background | This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2. [provided by RefSeq, Jul 2008] |
| Function | Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: Complement component antibodies; Complement component ELISA Kits; Complement component Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82648 Human Complement Factor B ELISA Kit standard curve image
ARG82648 Human Complement Factor B ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82648 Human Complement Factor B ELISA kit User manual |
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