ARG82723

Human Chymotrypsin ELISA Kit

Human Chymotrypsin ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82723-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82723-002 Standard 2 X 10 ng/vial 4°C
ARG82723-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82723-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82723-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82723-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82723-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82723-008 25X Wash buffer 20 ml 4°C
ARG82723-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82723-010 STOP solution 10 ml (Ready to use) 4°C
ARG82723-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82723 Human Chymotrypsin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Chymotrypsin in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name Chymotrypsin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 20 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.6%
Inter-Assay CV: 6.9%
Alternate Names ELA4; Chymotrypsin-C; CLCR; Caldecrin; EC 3.4.21.2

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11330 Human CTRC

Swiss-port # Q99895 Human Chymotrypsin-C

Gene Symbol CTRC
Gene Full Name chymotrypsin C (caldecrin)
Background This gene encodes a member of the peptidase S1 family. The encoded protein is a serum calcium-decreasing factor that has chymotrypsin-like protease activity. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]
Function Regulates activation and degradation of trypsinogens and procarboxypeptidases by targeting specific cleavage sites within their zymogen precursors. Has chymotrypsin-type protease activity and hypocalcemic activity. [UniProt]
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Images (1) Click the Picture to Zoom In

  • ARG82723 Human Chymotrypsin ELISA Kit standard curve image

    ARG82723 Human Chymotrypsin ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG82723 Human Chymotrypsin ELISA kit User manual download icon Download