ARG82803
Human Chromogranin A ELISA Kit
Human Chromogranin A ELISA Kit for ELISA and Human
Cancer kit; Controls and Markers kit; Neuroscience kit; Signaling Transduction kit
Overview
| Product Description | ARG82803 Human Chromogranin A ELISA Kit is an Enzyme Immunoassay kit for the quantification of Chromogranin A in Human serum. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Chromogranin A |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.4 µg/L |
| Sample Type | Serum |
| Standard Range | 30 - 900 µg/L |
| Sample Volume | 50 µl serum (diluted 1+8) |
| Precision | Intra-Assay CV: 4.9% Inter-Assay CV: 10.2% |
| Alternate Names | Vasostatin II; CGA; Vasostatin I; Pituitary secretory protein I; SP-I; CgA; SL21; Chromogranin-A |
Application Instructions
| Assay Time | 2 hours 30 min |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CHGA |
| Gene Full Name | chromogranin A |
| Background | The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014] |
| Function | Pancreastatin: Strongly inhibits glucose induced insulin release from the pancreas. Catestatin: Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed:15326220). Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed:15723172 and PubMed:24723458). Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed:21214543). Acts as a potent scavenger of free radicals in vitro (PubMed:24723458). May play a role in the regulation of cardiac function and blood pressure (PubMed:18541522). Serpinin: Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation. [UniProt] |
| Cellular Localization | Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle membrane. Secreted. Note=Associated with the secretory granule membrane through direct interaction to SCG3 that in turn binds to cholesterol-enriched lipid rafts in intragranular conditions. Serpinin: Secreted. Cytoplasmic vesicle, secretory vesicle. Note=Pyroglutaminated serpinin localizes to secretory vesicle. [UniProt] |
| Highlight | Related products: Chromogranin A antibodies; Chromogranin A ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cancer kit; Controls and Markers kit; Neuroscience kit; Signaling Transduction kit |
| PTM | Sulfated on tyrosine residues and/or contains sulfated glycans. O-glycosylated with core 1 or possibly core 8 glycans. Proteolytic processing gives rise to an additional longer form of catestatin (residues 358-390) which displays a less potent catecholamine release-inhibitory activity (PubMed:10781584). Plasmin-mediated proteolytic processing can give rise to additional shorter and longer forms of catestatin peptides (PubMed:17991725). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82803 Human Chromogranin A ELISA Kit standard curve image
ARG82803 Human Chromogranin A ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82803 Human Chromogranin A ELISA Kit User's manual |
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