ARG82692
Human Cathepsin L ELISA Kit
Human Cathepsin L ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82692-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82692-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82692-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82692-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82692-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82692-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82692-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82692-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82692-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82692-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82692-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82692 Human Cathepsin L ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Cathepsin L in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Cathepsin L |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 32 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.7% Inter-Assay CV: 5.6% |
| Alternate Names | Major excreted protein; Cathepsin L; CATL; Cathepsin L1; EC 3.4.22.15; CTSL1; MEP |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CTSL |
| Gene Full Name | cathepsin L |
| Background | The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2012] |
| Function | Important for the overall degradation of proteins in lysosomes. [UniProt] |
| Cellular Localization | Lysosome. [UniProt] |
| Highlight | Related products: Cathepsin antibodies; Cathepsin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82692 Human Cathepsin L ELISA Kit standard curve image
ARG82692 Human Cathepsin L ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82692 Human Cathepsin L ELISA kit User manual |
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