ARG82850
Human Cathepsin H ELISA Kit
Human Cathepsin H ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82850-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82850-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82850-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82850-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82850-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82850-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82850-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82850-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82850-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82850-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82850-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82850 Human Cathepsin H ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Cathepsin H in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Cathepsin H |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.2% Inter-Assay CV: 6.2% |
| Alternate Names | CPSB; EC 3.4.22.16; minichain; ACC5; ACC4; ACC-5; ACC-4; Pro-cathepsin H |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CTSH |
| Gene Full Name | cathepsin H |
| Background | The protein encoded by this gene is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016] |
| Function | Important for the overall degradation of proteins in lysosomes. [UniProt] |
| Cellular Localization | Lysosome. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82850 Human Cathepsin H ELISA Kit standard curve image
ARG82850 Human Cathepsin H ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82850 Human Cathepsin H ELISA Kit User manual |
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