ARG81433
Human Cathepsin D ELISA Kit
Human Cathepsin D ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81433-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81433-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81433-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81433-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81433-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81433-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81433-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81433-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81433-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81433-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81433-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81433 Human Cathepsin D ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Cathepsin D in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Cathepsin D |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.5% Inter-Assay CV: 7.5% |
| Alternate Names | CPSD; EC 3.4.23.5; HEL-S-130P; CLN10; Cathepsin D |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CTSD |
| Gene Full Name | cathepsin D |
| Background | This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease. [provided by RefSeq, Jul 2008] |
| Function | Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. [UniProt] |
| Highlight | Related products: Cathepsin antibodies; Cathepsin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N- and O-glycosylated. Undergoes proteolytic cleavage and activation by ADAM30. As well as the major heavy chain which starts at Leu-169, 2 minor forms starting at Gly-170 and Gly-171 have been identified (PubMed:1426530). An additional form starting at Ala-168 has also been identified (PubMed:27333034). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81433 Human Cathepsin D ELISA Kit standard curve image
ARG81433 Human Cathepsin D ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81433 Human Cathepsin D ELISA Kit User's manual |
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