ARG81433

Human Cathepsin D ELISA Kit

Human Cathepsin D ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81433-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81433-002 Standard 2 X 10 ng/vial 4°C
ARG81433-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81433-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81433-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81433-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81433-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81433-008 25X Wash buffer 20 ml 4°C
ARG81433-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81433-010 STOP solution 10 ml (Ready to use) 4°C
ARG81433-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81433 Human Cathepsin D ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Cathepsin D in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Cathepsin D
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.5%
Inter-Assay CV: 7.5%
Alternate Names CPSD; EC 3.4.23.5; HEL-S-130P; CLN10; Cathepsin D

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1509 Human CTSD

Swiss-port # P07339 Human Cathepsin D

Gene Symbol CTSD
Gene Full Name cathepsin D
Background This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease. [provided by RefSeq, Jul 2008]
Function Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. [UniProt]
Highlight Related products:
Cathepsin antibodies; Cathepsin ELISA Kits;
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Simplify the ELISA analysis by GainData
PTM N- and O-glycosylated.

Undergoes proteolytic cleavage and activation by ADAM30.

As well as the major heavy chain which starts at Leu-169, 2 minor forms starting at Gly-170 and Gly-171 have been identified (PubMed:1426530). An additional form starting at Ala-168 has also been identified (PubMed:27333034). [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG81433 Human Cathepsin D ELISA Kit standard curve image

    ARG81433 Human Cathepsin D ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81433 Human Cathepsin D ELISA Kit User's manual download icon Download