ARG81432
Human Cathepsin B ELISA Kit
Human Cathepsin B ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81432-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81432-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81432-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81432-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81432-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81432-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81432-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81432-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81432-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81432-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81432-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81432 Human Cathepsin B ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Cathepsin B in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Cathepsin B |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 7.2% Inter-Assay CV: 8.3% |
| Alternate Names | EC 3.4.22.1; Cathepsin B; APPS; CPSB; APP secretase; Cathepsin B1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CTSB |
| Gene Full Name | cathepsin B |
| Background | The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer disease, the most common cause of dementia. Overexpression of the encoded protein, which is a member of the peptidase C1 family, has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. [UniProt] |
| Highlight | Related products: Cathepsin antibodies; Cathepsin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81432 Human Cathepsin B ELISA Kit standard curve image
ARG81432 Human Cathepsin B ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81432 Human Cathepsin B ELISA Kit User's manual |
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