ARG82784
Human Caspase 3 ELISA Kit
Human Caspase 3 ELISA Kit for ELISA and Human
Publication1
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82784-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82784-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82784-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82784-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82784-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82784-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82784-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82784-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82784-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82784-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82784-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82784 Human Caspase 3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Caspase 3 in serum and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Caspase 3 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.1% Inter-Assay CV: 7.1% |
| Alternate Names | CPP-32; Caspase-3; EC 3.4.22.56; Apopain; CASP-3; CPP32; Cysteine protease CPP32; SCA-1; SREBP cleavage activity 1; CPP32B; Protein Yama |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CASP3 |
| Gene Full Name | caspase 3, apoptosis-related cysteine peptidase |
| Background | The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017] |
| Function | Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| PTM | Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa. S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82784 Human Caspase 3 (total) ELISA Kit standard curve image
ARG82784 Human Caspase 3 (total) ELISA Kit results of a typical standard run with optical density reading at 450 nm.
Specific References
Oral Galvanism Side Effects: Comparing Alloy Ions and Galvanic Current Effects on the Mucosa-like Model
ELISA / Human / Cell culture supernatant
| Title | Download Link |
|---|---|
| ARG82784 Human Caspase 3 ELISA Kit User manual |
 Download
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