ARG82618
Human/Canine/Porcine Insulin ELISA Kit
Human/Canine/Porcine Insulin ELISA Kit for ELISA and Human,Dog,Pig
Overview
| Product Description | ARG82618 Human/Canine/Porcine Insulin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Insulin in serum, plasma and cell culture supernatants. |
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| Tested Reactivity | Hu, Dog, Pig |
| Tested Application | ELISA |
| Specificity | This kit could assay both natural and recombinant Human/Canine/Porcine Insulin. No significant cross-reactivity or interference was observed in the following samples: Human: C-peptide, IGF1, IGF2, IL2, IL4, IL6, IL8, IL10, IL12, IL18, IL22, Insulin R, MCP1, Relaxin 1, Relaxin 2, Relaxin 3, TGF beta 1, TNF alpha and VEGF. Mouse: GM-CSF, IFN gamma, IL1 beta, IL2, IL4, IL6, IL10, IL17A and TNF alpha. Rat: IFN gamma, IL1 beta, IL4, IL6, IL10 and TNF alpha. The cross-reactivity to human proinsulin is 4.7%. The reactivity to Canine and Porcine insulins are predicted to be worked based on sequence homology. |
| Target Name | Insulin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 3.9 pmol/L |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 7.8 - 500 pmol/L |
| Sample Volume | 50 µl |
| Precision | Intra-Assay CV: 4.5% Inter-Assay CV: 4.2% |
| Alternate Names | IDDM; IDDM2; IDDM1; ILPR; MODY10; Insulin; IRDN |
Application Instructions
| Assay Time | ~ 2.5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | INS |
| Gene Full Name | insulin |
| Background | After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010] |
| Function | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: Insulin antibodies; Insulin ELISA Kits; Insulin Duos / Panels; Insulin recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82618 Human/Canine/Porcine Insulin ELISA Kit standard curve image
ARG82618 Human/Canine/Porcine Insulin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82618 Human/Canine/Porcine Insulin ELISA kit User manual |
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