ARG82576
Human Cadherin 15 ELISA Kit
Human Cadherin 15 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82576-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82576-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82576-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82576-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82576-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82576-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82576-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82576-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82576-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82576-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82576-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82576 Human Cadherin 15 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Cadherin 15 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Cadherin 15 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.3% Inter-Assay CV: 5.9% |
| Alternate Names | CDHM; CDH14; MRD3; MCAD; Cadherin-15; Cadherin-14; Muscle cadherin; M-cadherin; CDH3 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CDH15 |
| Gene Full Name | cadherin 15, type 1, M-cadherin (myotubule) |
| Background | This gene is a member of the cadherin superfamily of genes, encoding calcium-dependent intercellular adhesion glycoproteins. Cadherins consist of an extracellular domain containing 5 cadherin domains, a transmembrane region, and a conserved cytoplasmic domain. Transcripts from this particular cadherin are expressed in myoblasts and upregulated in myotubule-forming cells. The protein is thought to be essential for the control of morphogenetic processes, specifically myogenesis, and may provide a trigger for terminal muscle cell differentiation. [provided by RefSeq, Jul 2008] |
| Function | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| Highlight | Related products: Cadherin antibodies; Cadherin ELISA Kits; Cadherin Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82576 Human Cadherin 15 ELISA Kit standard curve image
ARG82576 Human Cadherin 15 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82576 Human Cadherin 15 ELISA Kit User manual |
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