ARG81523
Human CYR61 ELISA Kit
Human CYR61 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81523-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81523-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81523-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81523-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81523-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81523-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81523-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81523-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81523-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81523-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81523-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81523 Human CYR61 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CYR61 in serum, plasma (heparin, EDTA), saliva, urine, human milk and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CYR61 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), saliva, urine, human milk and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.0% Inter-Assay CV: 7.0% |
| Alternate Names | Protein GIG1; CCN1; IGFBP-10; Insulin-like growth factor-binding protein 10; IGFBP10; GIG1; Cysteine-rich angiogenic inducer 61; Protein CYR61; CCN family member 1; IGF-binding protein 10; IBP-10 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CYR61 |
| Gene Full Name | cysteine-rich, angiogenic inducer, 61 |
| Background | The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation. [provided by RefSeq, Sep 2011] |
| Function | Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. [UniProt] |
| Highlight | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81523 Human CYR61 ELISA Kit standard curve image
ARG81523 Human CYR61 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81523 Human CYR61 ELISA Kit User manual |
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