ARG81442

Human CXCL5 ELISA Kit

Human CXCL5 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81442-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81442-002 Standard 2 X 10 ng/vial 4°C
ARG81442-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81442-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81442-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81442-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81442-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81442-008 25X Wash buffer 20 ml 4°C
ARG81442-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81442-010 STOP solution 10 ml (Ready to use) 4°C
ARG81442-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81442 Human CXCL5 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CXCL5 in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CXCL5
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.2%
Inter-Assay CV: 7.3%
Alternate Names Neutrophil-activating peptide ENA-78; Epithelial-derived neutrophil-activating protein 78; SCYB5; Small-inducible cytokine B5; C-X-C motif chemokine 5; 9-78; 8-78; 1-78; ENA-78

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6374 Human CXCL5

Swiss-port # P42830 Human C-X-C motif chemokine 5

Gene Symbol CXCL5
Gene Full Name chemokine (C-X-C motif) ligand 5
Background This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils, to promote angiogenesis and to remodel connective tissues. This protein is thought to play a role in cancer cell proliferation, migration, and invasion. [provided by RefSeq, May 2013]
Function Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. [UniProt]
Highlight Related products:
CXCL antibodies; CXCL ELISA Kits; CXCL Duos / Panels; CXCL recombinant proteins;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM N-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG81442 Human CXCL5 ELISA Kit standard curve image

    ARG81442 Human CXCL5 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81442 Human CXCL5 ELISA Kit User manual download icon Download