ARG83027
Human CXCL1 / GRO alpha ELISA Kit
Human CXCL1 / GRO alpha ELISA Kit for ELISA and Human
Overview
| Product Description | ARG83027 Human CXCL1 / GRO alpha ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CXCL1 / GRO alpha in serum, plasma (heparin), ascites, urine and cell culture supernatants. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | Not cross-reacts with: Human GRO beta, IL8, RANTES, ENA78, MIP1 alpha, MIP1 beta, NAP2, MCP-1. Mouse KC, MIP1alpha, MIP1 beta, MIP2. Rat CINC1. |
| Target Name | CXCL1 / GRO alpha |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Protein Full Name | Growth-regulated alpha protein |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin), ascites, urine and cell culture supernatants. |
| Standard Range | 31.3 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: less than 10% Inter-Assay CV: less than 10% |
| Alternate Names | CXCL1, C X C Motif Chemokine Ligand , SCYB, NAP-3, MGSA A, GROa, GRO1, MGSA, Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha), GRO1 Oncogene (Melanoma Growth Stimulating Activity, Alpha), Melanoma Growth Stimulating Activity, Alpha, Neutrophil-Activating Protein 3, Growth-Regulated Alpha Protein, Fibroblast Secretory Protein, C-X-C Motif Chemokine , GRO-Alpha(1-73), FSP, GRO1 Oncogene (Melanoma Growth-Stimulating Activity), Melanoma Growth Stimulatory Activity Alpha, Melanoma Growth Stimulatory Activity, MGSA Alpha, GROA, GRO |
Application Instructions
| Assay Time | ~ 4 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | CXCL1 |
| Gene Full Name | C-X-C Motif Chemokine Ligand 1 |
| Background | This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression of this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4. [provided by RefSeq, Sep 2014] |
| Function | Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: CXCL1 antibodies; CXCL1 ELISA Kits; Related news: Immune signaling protein TLR4 has opposing roles in breast cancer development New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG83027 Human CXCL1 / GRO alpha ELISA Kit standard curve image
ARG83027 Human CXCL1 / GRO alpha ELISA Kit results of standard run with optical density reading at 450 nm
| Title | Download Link |
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| ARG83027 Human CXCL1 / GRO alpha ELISA Kit User manual |
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