ARG81631
Human CXCL1 / GRO alpha ELISA Kit
Human CXCL1 / GRO alpha ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81631-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81631-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81631-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81631-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81631-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81631-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81631-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81631-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81631-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81631-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81631-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81631 HumanCXCL1 / GRO alpha ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CXCL1 / GRO alpha in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with 1 ng/ml of human recombinant CXCL2 and CXCL3. |
| Target Name | CXCL1 / GRO alpha |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.2% Inter-Assay CV: 7.2% |
| Alternate Names | Growth-regulated alpha protein; SCYB1; Melanoma growth stimulatory activity; MGSA-a; GRO-alpha; GROa; NAP-3; FSP; 5-73; C-X-C motif chemokine 1; Neutrophil-activating protein 3; 6-73; 1-73; GRO1; MGSA; 4-73 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CXCL1 |
| Gene Full Name | chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) |
| Background | This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression of this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4. [provided by RefSeq, Sep 2014] |
| Function | Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. [UniProt] |
| Highlight | Related products: CXCL antibodies; CXCL ELISA Kits; CXCL Duos / Panels; CXCL recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81631 Human CXCL1 / GRO alpha ELISA Kit standard curve image
ARG81631 Human CXCL1 / GRO alpha ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81631 Human CXCL1 / GRO alpha ELISA Kit User's manual |
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