ARG82612
Human CTGF ELISA Kit
Human CTGF ELISA Kit for ELISA and Human
Publication1
Overview
| Product Description | ARG82612 Human CTGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CTGF in serum, plasma and cell culture supernatants. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | This kit could assay both natural and recombinant Human CTGF. No significant cross-reactivity or interference was observed in the following samples: Human: IFN gamma, IL1 beta, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL17A, IL18, IL21, IL22, IL23, MCP1, TGF beta 1, TNF alpha and VEGF. Mouse: IFN gamma, IL1 beta, IL2, IL4, IL6, IL10, IL17A and TNF alpha. Rat: IFN gamma, IL1 beta, IL4, IL6, IL10 and TNF alpha. |
| Target Name | CTGF |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.3 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 50 - 100 µl |
| Precision | Intra-Assay CV: 5.8% Inter-Assay CV: 6.9% |
| Alternate Names | Insulin-like growth factor-binding protein 8; IGFBP-8; CCN2; IBP-8; HCS24; Connective tissue growth factor; IGF-binding protein 8; CCN family member 2; IGFBP8; Hypertrophic chondrocyte-specific protein 24; NOV2 |
Application Instructions
| Assay Time | ~ 3.5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | CTGF |
| Gene Full Name | connective tissue growth factor |
| Background | The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq, Nov 2009] |
| Function | Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. [UniProt] |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. Secreted. [UniProt] |
| Highlight | Related products: CTGF antibodies; CTGF ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82612 Human CTGF ELISA Kit standard curve image
ARG82612 Human CTGF ELISA Kit results of a typical standard run with optical density reading at 450 nm.
Specific References
Hepatocyte growth factor exhibits anti-fibrotic effects in an in vitro model of nifedipine-induced gingival overgrowth
ELISA / Human / Cell culture supernatants
| Title | Download Link |
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| ARG82612 Human CTGF ELISA Kit User manual |
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