ARG82444
Human CST6 / Cystatin E ELISA Kit
Human CST6 / Cystatin E ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82444-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82444-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82444-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82444-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82444-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82444-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82444-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82444-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82444-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82444-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82444-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82444 Human CST6 / Cystatin E ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CST6 / Cystatin E in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | CST6 / Cystatin E |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 23.45 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 46.9 - 3000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.5% Inter-Assay CV: 5.7% |
| Alternate Names | Cystatin-E; Cystatin-6; Cystatin-M |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CST6 |
| Gene Full Name | cystatin E/M |
| Background | The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. This gene encodes a cystatin from the type 2 family, which is down-regulated in metastatic breast tumor cells as compared to primary tumor cells. Loss of expression is likely associated with the progression of a primary tumor to a metastatic phenotype. [provided by RefSeq, Jul 2008] |
| Function | Shows moderate inhibition of cathepsin B but is not active against cathepsin C. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: Cystatin antibodies; Cystatin ELISA Kits; Cystatin Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Substrate for transglutaminases. Acts as an acyl acceptor but not as an acyl donor. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82444 Human CST6 / Cystatin E ELISA Kit standard curve image
ARG82444 Human CST6 / Cystatin E ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82444 Human CST6 / Cystatin E ELISA Kit User manual |
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