ARG82443
Human CST5 / Cystatin D ELISA Kit
Human CST5 / Cystatin D ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82443-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82443-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82443-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82443-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82443-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82443-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82443-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82443-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82443-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82443-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82443-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82443 Human CST5 / Cystatin D ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CST5 / Cystatin D in serum, plasma (EDTA, heparin), cell culture supernatants, saliva and urine. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | CST5 / Cystatin D |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 10 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin), cell culture supernatants, saliva and urine. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.1% Inter-Assay CV: 6.1% |
| Alternate Names | Cystatin-D; Cystatin-5 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CST5 |
| Gene Full Name | cystatin D |
| Background | The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein found in saliva and tears. The encoded protein may play a protective role against proteinases present in the oral cavity. [provided by RefSeq, Jul 2008] |
| Function | Cysteine proteinase inhibitor that possibly plays a protective role against proteinases present in the oral cavity. The order of preference for inhibition is cathepsin S > cathepsin H > cathepsin L > cathepsin B. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: Cystatin antibodies; Cystatin ELISA Kits; Cystatin Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82443 Human CST5 / Cystatin D ELISA Kit standard curve image
ARG82443 Human CST5 / Cystatin D ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82443 Human CST5 / Cystatin D ELISA Kit User manual |
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