ARG81549
Human CRLF2 ELISA Kit
Human CRLF2 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81549-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81549-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81549-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81549-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81549-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81549-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81549-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81549-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81549-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81549-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81549-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81549 Human CRLF2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CRLF2 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CRLF2 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.3 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.7% Inter-Assay CV: 6.9% |
| Alternate Names | CRL2; Cytokine receptor-like 2; Cytokine receptor-like factor 2; Thymic stromal lymphopoietin protein receptor; TSLP receptor; TSLPR; IL-XR; CRLF2Y |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CRLF2 |
| Gene Full Name | cytokine receptor-like factor 2 |
| Background | This gene encodes a member of the type I cytokine receptor family. The encoded protein is a receptor for thymic stromal lymphopoietin (TSLP). Together with the interleukin 7 receptor (IL7R), the encoded protein and TSLP activate STAT3, STAT5, and JAK2 pathways, which control processes such as cell proliferation and development of the hematopoietic system. Rearrangement of this gene with immunoglobulin heavy chain gene (IGH) on chromosome 14, or with P2Y purinoceptor 8 gene (P2RY8) on the same X or Y chromosomes is associated with B-progenitor acute lymphoblastic leukemia (ALL) and Down syndrome ALL. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014] |
| Function | Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2 (By similarity). Implicated in the development of the hematopoietic system. [UniProt] |
| Highlight | Related products: CRLF2 antibodies; CRLF2 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81549 Human CRLF2 ELISA Kit standard curve image
ARG81549 Human CRLF2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81549 Human CRLF2 ELISA Kit User's manual |
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