ARG81914
Human COMT ELISA Kit
Human COMT ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81914-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81914-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81914-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81914-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81914-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81914-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81914-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81914-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81914-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81914-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81914-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81914 Human COMT ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human COMT in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | COMT |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.8%; Inter-Assay CV: 7.9% |
| Alternate Names | EC 2.1.1.6; Catechol O-methyltransferase; HEL-S-98n |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | COMT |
| Gene Full Name | catechol-O-methyltransferase |
| Background | Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008] |
| Function | Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. [UniProt] |
| Highlight | Related products: COMT antibodies; COMT ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The N-terminus is blocked. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81914 Human COMT ELISA Kit standard curve image
ARG81914 Human COMT ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81914 Human COMT ELISA Kit User manual |
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