ARG83451

Human CKM / Creatine Kinase M ELISA Kit

Human CKM / Creatine Kinase M ELISA Kit for ELISA and Human

Overview

Product Description ARG83451 Human CKM / Creatine Kinase M ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CKM / Creatine Kinase M in Serum, plasma and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name CKM / Creatine Kinase M
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.1 mU/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 0.15 - 10 mU/ml
Sample Volume 100 µl
Precision Intra-Assay CV: < 10%
Inter-Assay CV: < 10%
Alternate Names CKMM; M-CK; Creatine kinase M chain; EC 2.7.3.2; Creatine kinase M-type

Application Instructions

Assay Time 3.5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 4°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1158 Human CKM

Swiss-port # P06732 Human Creatine kinase M-type

Gene Symbol CKM
Gene Full Name creatine kinase, muscle
Background The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]
Function Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG83451 Human CKM / Creatine Kinase M ELISA Kit standard curve image

    ARG83451 Human CKM / Creatine Kinase M ELISA Kit results of a typical standard run with optical density reading at 450 nm.

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