ARG82456
Human CILP ELISA Kit
Human CILP ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82456-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82456-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82456-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82456-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82456-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82456-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82456-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82456-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82456-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82456-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82456-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82456 Human CILP ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CILP in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | CILP |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 50 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 93.8 - 6000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.4% Inter-Assay CV: 5.9% |
| Alternate Names | Cartilage intermediate layer protein 1; Cartilage intermediate-layer protein; CILP-1; HsT18872 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O75339 Human Cartilage intermediate layer protein 1 |
|---|---|
| Gene Symbol | CILP |
| Gene Full Name | cartilage intermediate layer protein, nucleotide pyrophosphohydrolase |
| Background | Major alterations in the composition of the cartilage extracellular matrix occur in joint disease, such as osteoarthrosis. This gene encodes the cartilage intermediate layer protein (CILP), which increases in early osteoarthrosis cartilage. The encoded protein was thought to encode a protein precursor for two different proteins; an N-terminal CILP and a C-terminal homolog of NTPPHase, however, later studies identified no nucleotide pyrophosphatase phosphodiesterase (NPP) activity. The full-length and the N-terminal domain of this protein was shown to function as an IGF-1 antagonist. An allelic variant of this gene has been associated with lumbar disc disease. [provided by RefSeq, Sep 2010] |
| Function | Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage. [UniProt] |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. [UniProt] |
| Highlight | Related products: CILP antibodies; CILP ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Cleaved into 2 chains possibly by a furin-like protease upon or preceding secretion. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82456 Human CILP ELISA Kit standard curve image
ARG82456 Human CILP ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82456 Human CILP ELISA Kit User manual |
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