ARG82486
Human CHIT1 ELISA Kit
Human CHIT1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82486-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82486-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82486-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82486-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82486-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82486-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82486-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82486-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82486-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82486-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82486-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82486 Human CHIT1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CHIT1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | CHIT1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 156 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 312 - 20000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.5% Inter-Assay CV: 6.3% |
| Alternate Names | EC 3.2.1.14; Chitotriosidase-1; CHI3; CHIT; Chitinase-1; CHITD |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CHIT1 |
| Gene Full Name | chitinase 1 (chitotriosidase) |
| Background | Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2012] |
| Function | Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity. [UniProt] |
| Cellular Localization | Secreted. Lysosome. Note=A small proportion is lysosomal. [UniProt] |
| Highlight | Related products: CHIT1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82486 Human CHIT1 ELISA Kit standard curve image
ARG82486 Human CHIT1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82486 Human CHIT1 ELISA Kit User manual |
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