ARG70391
Human CHI3L1 recombinant protein (His-tagged, C-ter)
Human CHI3L1 recombinant protein (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Human CHI3L1 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | CHI3L1 |
| Species | Human |
| A.A. Sequence | Tyr22 - Thr383 |
| Expression System | E. coli |
| Protein Full Name | Chitinase-3-like protein 1 |
| Alternate Names | CHI3L1; Chitinase 3 Like 1; YKL40 YK-40; GP39; Chitinase 3-Like 1 (Cartilage Glycoprotein-39); Chitinase-3-Like Protein 1; Cartilage Glycoprotein 39; 39 KDa Synovial Protein; HCGP-39; CGP-39; YKL-40; GP-39; Cartilage Glycoprotein-39; HC-Gp39; HCGP-3P; YYL-40; ASRT7 |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | >98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not <200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CHI3L1 |
|---|---|
| Gene Full Name | Chitinase 3 Like 1 |
| Background | Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. |
| Function | Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung. |
| Cellular Localization | Cytoplasm, Endoplasmic reticulum, Secreted |
| PTM | Disulfide bond, Glycoprotein |
Images (1) Click the Picture to Zoom In
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ARG70391 Human CHI3L1 recombinant protein (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70391 Human CHI3L1 recombinant protein (His-tagged, C-ter)
