ARG81494
Human CHI3L1 ELISA Kit
Human CHI3L1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81494-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81494-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81494-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81494-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81494-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81494-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81494-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81494-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81494-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81494-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81494-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81494 Human CHI3L1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CHI3L1 in serum, plasma (heparin, EDTA), urine and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CHI3L1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), urine and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.0% Inter-Assay CV: 6.6% |
| Alternate Names | YYL-40; GP-39; ASRT7; YKL40; HC-gp39; HCGP-3P; GP39; Cartilage glycoprotein 39; CGP-39; Chitinase-3-like protein 1; hCGP-39; YKL-40; 39 kDa synovial protein |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CHI3L1 |
| Gene Full Name | chitinase 3-like 1 (cartilage glycoprotein-39) |
| Background | Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009] |
| Function | Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung. [UniProt] |
| Highlight | Related products: CHI3L1 antibodies; CHI3L1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81494 Human CHI3L1 ELISA Kit standard curve image
ARG81494 Human CHI3L1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81494 Human CHI3L1 ELISA Kit User's manual |
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