ARG82420
Human CFHR5 ELISA Kit
Human CFHR5 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82420-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82420-002 | Standard | 2 X 50 ng/vial | 4°C |
| ARG82420-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82420-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82420-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82420-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82420-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82420-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82420-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82420-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82420-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82420 Human CFHR5 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CFHR5 in serum, plasma (EDTA, heparin), cell culture supernatants and urine. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | CFHR5 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.39 ng/ml |
| Sample Type | Serum, plasma (EDTA, heparin), cell culture supernatants and urine. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.5% Inter-Assay CV: 5.1% |
| Alternate Names | CFHL5; FHR-5; CFHR5D; FHR5; Complement factor H-related protein 5 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9BXR6 Human Complement factor H-related protein 5 |
|---|---|
| Gene Symbol | CFHR5 |
| Gene Full Name | complement factor H-related 5 |
| Background | This gene is a member of a small complement factor H (CFH) gene cluster on chromosome 1. Each member of this gene family contains multiple short consensus repeats (SCRs) typical of regulators of complement activation. The protein encoded by this gene has nine SCRs with the first two repeats having heparin binding properties, a region within repeats 5-7 having heparin binding and C reactive protein binding properties, and the C-terminal repeats being similar to a complement component 3 b (C3b) binding domain. This protein co-localizes with C3, binds C3b in a dose-dependent manner, and is recruited to tissues damaged by C-reactive protein. Allelic variations in this gene have been associated, but not causally linked, with two different forms of kidney disease: membranoproliferative glomerulonephritis type II (MPGNII) and hemolytic uraemic syndrome (HUS). [provided by RefSeq, Jan 2010] |
| Function | Involved in complement regulation. The dimerized forms have avidity for tissue-bound complement fragments and efficiently compete with the physiological complement inhibitor CFH. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
-
ARG82420 Human CFHR5 ELISA Kit standard curve image
ARG82420 Human CFHR5 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82420 Human CFHR5 ELISA Kit User manual |
 Download
|
