ARG82709
Human CES1 ELISA Kit
Human CES1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82709-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82709-002 | Standard | 2 X 50 ng/vial | 4°C |
| ARG82709-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82709-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82709-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82709-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82709-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82709-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82709-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82709-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82709-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82709 Human CES1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CES1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | CES1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.39 ng/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 0.78 - 50 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.6% Inter-Assay CV: 6.3% |
| Alternate Names | SES1; Cocaine carboxylesterase; REH; ACAT; Serine esterase 1; HMSE; Retinyl ester hydrolase; Methylumbelliferyl-acetate deacetylase 1; hCE-1; EC 3.1.1.56; CE-1; CEH; HMSE1; TGH; PCE-1; Acyl-coenzyme A:cholesterol acyltransferase; EC 3.1.1.1; Liver carboxylesterase 1; Brain carboxylesterase hBr1; Triacylglycerol hydrolase; CES2; Egasyn; Carboxylesterase 1; Monocyte/macrophage serine esterase |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CES1 |
| Gene Full Name | carboxylesterase 1 |
| Background | This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
| Function | Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate. [UniProt] |
| Cellular Localization | Endoplasmic reticulum lumen. [UniProt] |
| Highlight | Related products: CES antibodies; CES ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Contains sialic acid. Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82709 Human CES1 ELISA Kit standard curve image
ARG82709 Human CES1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82709 Human CES1 ELISA Kit User manual |
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