ARG81627
Human CDCP1 ELISA Kit
Human CDCP1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81627-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81627-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81627-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81627-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81627-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81627-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81627-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81627-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81627-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81627-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81627-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81627 Human CDCP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CDCP1 in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CDCP1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.4% Inter-Assay CV: 7.0% |
| Alternate Names | Membrane glycoprotein gp140; CD antigen CD318; SIMA135; Subtractive immunization M plus HEp3-associated 135 kDa protein; CUB domain-containing protein 1; TRASK; Transmembrane and associated with src kinases; CD318 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CDCP1 |
| Gene Full Name | CUB domain containing protein 1 |
| Background | This gene encodes a transmembrane protein which contains three extracellular CUB domains and acts as a substrate for Src family kinases. The protein plays a role in the tyrosine phosphorylation-dependent regulation of cellular events that are involved in tumor invasion and metastasis. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013] |
| Function | May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis. [UniProt] |
| Highlight | Related products: CDCP1 antibodies; CDCP1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Phosphorylated on tyrosine by kinases of the SRC family such as SRC and YES as well as by the protein kinase C gamma/PRKCG. Dephosphorylated by phosphotyrosine phosphatases. Also phosphorylated by suramin, a heparin analog. Tyrosine phosphorylated in response to dissociation of integrin alpha-6 beta-4 from laminin-5. N-glycosylated. A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). Another peptide of 80 kDa (p80) is present in cultured keratinocytes probably due to tryptic cleavage at an unidentified site on its N-terminal side. Converted to p80 by plasmin, a trypsin-like protease. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81627 Human CDCP1 ELISA Kit standard curve image
ARG81627 Human CDCP1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81627 Human CDCP1 ELISA Kit User manual |
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