ARG81572
Human CD97 ELISA Kit
Human CD97 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81572-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81572-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81572-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81572-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81572-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81572-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81572-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81572-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81572-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81572-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81572-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81572 Human CD97 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD97 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD97 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 62.5 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 125 - 8000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.3% Inter-Assay CV: 6.1% |
| Alternate Names | CD97; Leukocyte antigen CD97; CD97 antigen; CD antigen CD97; TM7LN1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ADGRE5 |
| Gene Full Name | adhesion G protein-coupled receptor E5 |
| Background | This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011] |
| Function | Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration (By similarity). [UniProt] |
| Highlight | Related products: CD97 antibodies; CD97 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81572 Human CD97 ELISA Kit standard curve image
ARG81572 Human CD97 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81572 Human CD97 ELISA Kit User's manual |
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