ARG82756
Human CD85j / LIR1 ELISA Kit
Human CD85j / LIR1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82756-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82756-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82756-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82756-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82756-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82756-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82756-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82756-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82756-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82756-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82756-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82756 Human CD85j / LIR1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD85j / LIR1 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | CD85j / LIR1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | less than 30 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.3% Inter-Assay CV: 6.4% |
| Alternate Names | LIR-1; Leukocyte immunoglobulin-like receptor 1; ILT2; CD85 antigen-like family member J; Immunoglobulin-like transcript 2; LIR1; ILT-2; Leukocyte immunoglobulin-like receptor subfamily B member 1; Monocyte/macrophage immunoglobulin-like receptor 7; CD antigen CD85j; CD85J; MIR7; MIR-7 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8NHL6 Human Leukocyte immunoglobulin-like receptor subfamily B member 1 |
|---|---|
| Gene Symbol | LILRB1 |
| Gene Full Name | leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1 |
| Background | This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Isoform 5: Secreted. [UniProt] |
| Highlight | Related products: CD85 antibodies; CD85 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Phosphorylated on tyrosine residues. Dephosphorylated by PTPN6. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82756 Human CD85j / LIR1 ELISA Kit standard curve image
ARG82756 Human CD85j / LIR1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82756 Human CD85j / LIR1 ELISA kit User manual |
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