ARG82763
Human CD85e / LILRA3 ELISA Kit
Human CD85e / LILRA3 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82763-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82763-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82763-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82763-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82763-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82763-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82763-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82763-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82763-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82763-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82763-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82763 Human CD85e / LILRA3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD85e / LILRA3 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | CD85e / LILRA3 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 156 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 312 - 20000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.1% Inter-Assay CV: 8.2% |
| Alternate Names | ILT6; LIR-4; CD85 antigen-like family member E; ILT-6; Immunoglobulin-like transcript 6; LIR4; HM31; HM43; Monocyte inhibitory receptor HM43/HM31; CD85E; CD antigen CD85e; Leukocyte immunoglobulin-like receptor subfamily A member 3; Leukocyte immunoglobulin-like receptor 4 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8N6C8 Human Leukocyte immunoglobulin-like receptor subfamily A member 3 |
|---|---|
| Gene Symbol | LILRA3 |
| Gene Full Name | leukocyte immunoglobulin-like receptor, subfamily A (without TM domain), member 3 |
| Background | This gene encodes a member of a family of immunoreceptors that are expressed predominantly in monocytes and B cells, and at lower levels in dendritic cells and natural killer cells. The encoded protein lacks the transmembrane region found in other members of this family. It acts as a soluble receptor for class I major histocompatibility complex (MHC) antigens. Alternatively spliced transcript variants encoding different isoforms have been found. This gene is located in a cluster of related genes on chromosome 19 and is polymorphic in human populations, with many individuals containing a deletion of this genomic region. [provided by RefSeq, Mar 2014] |
| Function | Acts as soluble receptor for class I MHC antigens. Binds both classical and non-classical HLA class I molecules but with reduced affinities compared to LILRB1 or LILRB2. Binds with high affinity to the surface of monocytes, leading to abolish LPS-induced TNF-alpha production by monocytes. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: CD85 antibodies; CD85 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N-glycosylation is required for ligand binding. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82763 Human CD85e / LILRA3 ELISA Kit standard curve image
ARG82763 Human CD85e / LILRA3 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82763 Human CD85e / LILRA3 ELISA Kit User manual |
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