ARG81718
Human CD80 ELISA Kit
Human CD80 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81718-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81718-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81718-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81718-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81718-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81718-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81718-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81718-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81718-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81718-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81718-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81718 Human CD80 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD80 in serum and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD80 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.5%; Inter-Assay CV: 6.2% |
| Alternate Names | B7.1; CTLA-4 counter-receptor B7.1; CD28LG; T-lymphocyte activation antigen CD80; B7-1; CD28LG1; B7; LAB7; Activation B7-1 antigen; CD antigen CD80; BB1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P33681 Human T-lymphocyte activation antigen CD80 |
|---|---|
| Gene Symbol | CD80 |
| Gene Full Name | CD80 molecule |
| Background | The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011] |
| Function | Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. [UniProt] |
| Highlight | Related products: CD80 antibodies; CD80 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81718 Human CD80 ELISA Kit standard curve image
ARG81718 Human CD80 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81718 Human CD80 ELISA Kit User's manual |
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