ARG83238
Human CD74 ELISA Kit
Human CD74 ELISA Kit for ELISA and Human
Immune System antibody
Overview
| Product Description | ARG83238 Human CD74 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Cd74 in Serum, Plasma and Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD74 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15 pg/ml |
| Detection Range | 156 pg/ml - 10,000 pg/ml |
| Sample Type | Serum, Plasma and Cell culture supernatants |
| Precision | Intra-Assay CV: 5.5% Inter-Assay CV: 6.5% |
| Alternate Names | DHLAG; Ii; CD antigen CD74; II; HLA-DR antigens-associated invariant chain; Ia-GAMMA; Ia antigen-associated invariant chain; HLA class II histocompatibility antigen gamma chain; p33; HLADG |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P04233 Human HLA class II histocompatibility antigen gamma chain |
|---|---|
| Gene Symbol | CD74 |
| Gene Full Name | CD74 molecule, major histocompatibility complex, class II invariant chain |
| Background | The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011] |
| Function | Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. [UniProt] |
| Cellular Localization | Cell membrane |
| Research Area | Immune System antibody |
| PTM | N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. |
Images (1) Click the Picture to Zoom In
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ARG83238 Human CD74 ELISA Kit standard curve image
ARG83238 Human CD74 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83238 Human CD74 ELISA Kit User manual |
 Download
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