ARG81920
Human CD73 ELISA Kit
Human CD73 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81920-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81920-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81920-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81920-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81920-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81920-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81920-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81920-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81920-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81920-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81920-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81920 Human CD73 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD73 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD73 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.0%; Inter-Assay CV: 7.0% |
| Alternate Names | 5'-nucleotidase; CD antigen CD73; eN; CALJA; CD73; EC 3.1.3.5; NT; Ecto-5'-nucleotidase; NT5; E5NT; 5'-NT; eNT; NTE |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NT5E |
| Gene Full Name | 5'-nucleotidase, ecto (CD73) |
| Background | The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011] |
| Function | Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. [UniProt] |
| Highlight | Related products: CD73 antibodies; CD73 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81920 Human CD73 ELISA Kit standard curve image
ARG81920 Human CD73 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81920 Human CD73 ELISA Kit User manual |
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