ARG81564
Human CD66a / CEACAM1 ELISA Kit
Human CD66a / CEACAM1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81564-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81564-002 | Standard | 2 X 20 ng/vial | 4°C |
| ARG81564-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81564-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81564-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81564-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81564-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81564-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81564-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81564-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81564-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81564 Human CD66a / CEACAM1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD66a / CEACAM1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD66a / CEACAM1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 156 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 312 - 20000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.7% Inter-Assay CV: 6.8% |
| Alternate Names | Carcinoembryonic antigen-related cell adhesion molecule 1; BGP-1; BGP1; Biliary glycoprotein 1; BGP; BGPI; CD antigen CD66a |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P13688 Human Carcinoembryonic antigen-related cell adhesion molecule 1 |
|---|---|
| Gene Symbol | CEACAM1 |
| Gene Full Name | carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) |
| Background | This gene encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature of all variants has not been defined. [provided by RefSeq, May 2010] |
| Highlight | Related products: CD66 antibodies; CD66 ELISA Kits; CD66 Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Isoform 1: Phosphorylated on serine and tyrosine (By similarity). Isoform 1 is phosphorylated on tyrosine by Src family kinases like SRC and LCK and by receptor like CSF3R, EGFR and INSR upon stimulation (PubMed:15467833, PubMed:18424730, PubMed:7478590). Phosphorylated at Ser-508; mediates activity. Phosphorylated at Tyr-493; regulates activity (By similarity). Phosphorylated at Tyr-493 by EGFR and INSR upon stimulation; this phosphorylation is Ser-508-phosphorylation-dependent; mediates cellular internalization; increases interaction with downstream proteins like SHC1 and FASN (By similarity). Phosphorylated at Tyr-493 and Tyr-520 by LCK; mediates PTPN6 association and is regulated by homophilic ligation of CEACAM1 in the absence of T cell activation (PubMed:18424730). Phosphorylated at Tyr-520; mediates interaction with PTPN11 (By similarity). Isoform 8: Phosphorylated on serine and threonine. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81564 Human CD66a / CEACAM1 ELISA Kit standard curve image
ARG81564 Human CD66a / CEACAM1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81564 Human Cd66a ELISA Kit User's manual |
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