ARG82431
Human CD55 ELISA Kit
Human CD55 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82431-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82431-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82431-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82431-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82431-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82431-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82431-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82431-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82431-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82431-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82431-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82431 Human CD55 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD55 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | CD55 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 156 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 312 - 20000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.2% Inter-Assay CV: 6.1% |
| Alternate Names | DAF; CD antigen CD55; CROM; Complement decay-accelerating factor; CR; TC |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P08174 Human Complement decay-accelerating factor |
|---|---|
| Gene Symbol | CD55 |
| Gene Full Name | CD55 molecule, decay accelerating factor for complement (Cromer blood group) |
| Background | This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. [provided by RefSeq, Jul 2014] |
| Function | This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. [UniProt] |
| Cellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Cell membrane; Lipid-anchor, GPI-anchor. Isoform 3: Secreted. Isoform 4: Secreted. Isoform 5: Secreted. Isoform 6: Cell membrane; Lipid-anchor, GPI-anchor. Isoform 7: Cell membrane; Lipid-anchor, GPI-anchor. [UniProt] |
| Highlight | Related products: CD55 antibodies; CD55 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The Ser/Thr-rich domain is heavily O-glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82431 Human CD55 ELISA Kit standard curve image
ARG82431 Human CD55 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82431 Human CD55 ELISA Kit User manual |
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