ARG81940
Human CD328 / SIGLEC7 ELISA Kit
Human CD328 / SIGLEC7 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81940-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81940-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81940-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81940-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81940-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81940-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81940-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81940-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81940-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81940-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81940-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81940 Human CD328 / SIGLEC7 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD328 / SIGLEC7 in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD328 / Siglec 7 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 62.5 pg/ml |
| Sample Type | Serum, plasma (heparin) and cell culture supernatants. |
| Standard Range | 125 - 8000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.0%; Inter-Assay CV: 6.6% |
| Alternate Names | QA79 membrane protein; p75; CD antigen CD328; SIGLEC19P; Adhesion inhibitory receptor molecule 1; SIGLECP2; Sialic acid-binding Ig-like lectin 7; SIGLEC-7; p75/AIRM1; CDw328; Siglec-7; AIRM-1; CD328; AIRM1; D-siglec; QA79 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9Y286 Human Sialic acid-binding Ig-like lectin 7 |
|---|---|
| Gene Symbol | SIGLEC7 |
| Gene Full Name | sialic acid binding Ig-like lectin 7 |
| Function | Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killer cells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro). [UniProt] |
| Highlight | Related products: CD328 antibodies; CD328 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Tyrosine phosphorylated. [UniProt] |
Images (1) Click the Picture to Zoom In
-
ARG81940 Human CD328 / SIGLEC7 ELISA Kit standard curve image
ARG81940 Human CD328 / SIGLEC7 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81940 Human CD328 / SIGLEC7 ELISA Kit User's manual |
 Download
|
