ARG81895
Human CD321 / JAM1 ELISA Kit
Human CD321 / JAM1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81895-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81895-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81895-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81895-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81895-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81895-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81895-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81895-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81895-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81895-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81895-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81895 Human CD321 / JAM1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD321 / JAM1 in serum and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD321 / JAM1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 23.45 pg/ml |
| Sample Type | Serum and cell culture supernatants. |
| Standard Range | 46.9 - 3000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.2%; Inter-Assay CV: 6.8% |
| Alternate Names | JAM-1; JAM1; Junctional adhesion molecule A; CD antigen CD321; JCAM; JAM-A; Junctional adhesion molecule 1; PAM-1; JAM; JAMA; KAT; Platelet F11 receptor; Platelet adhesion molecule 1; CD321 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | F11R |
| Gene Full Name | F11 receptor |
| Background | Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established. [provided by RefSeq, Jul 2008] |
| Function | Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus. [UniProt] |
| Highlight | Related products: CD321 antibodies; CD321 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N-glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81895 Human CD321 / JAM1 ELISA Kit standard curve image
ARG81895 Human CD321 / JAM1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81895 Human CD321 / JAM1 ELISA Kit User's manual |
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